
                                SEQUENCE LISTING

<110> Kloti, Andreas
      Woessner, Jeffrey
      Zayed, Adel
      Gorlach, Jorn
      Boyes, Doug
      Davis, Keith
      Hamilton, Carol
      Ascenzi, Robert
      Hoffman, Neil

<120> METHODS FOR THE IDENTIFICATION OF
  INHIBITORS OF CYSTEINE SYNTHASE EXPRESSION OR ACTIVITY IN
  PLANTS

<130> 2040 US

<160> 6

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 322
<212> PRT
<213> Arabiodopsis thaliana

<400> 1
Met Ala Ser Arg Ile Ala Lys Asp Val Thr Glu Leu Ile Gly Asn Thr
 1               5                  10                  15      
Pro Leu Val Tyr Leu Asn Asn Val Ala Glu Gly Cys Val Gly Arg Val
            20                  25                  30          
Ala Ala Lys Leu Glu Met Met Glu Pro Cys Ser Ser Val Lys Asp Arg
        35                  40                  45              
Ile Gly Phe Ser Met Ile Ser Asp Ala Glu Lys Lys Gly Leu Ile Lys
    50                  55                  60                  
Pro Gly Glu Ser Val Leu Ile Glu Pro Thr Ser Gly Asn Thr Gly Val
65                  70                  75                  80  
Gly Leu Ala Phe Thr Ala Ala Ala Lys Gly Tyr Lys Leu Ile Ile Thr
                85                  90                  95      
Met Pro Ala Ser Met Ser Thr Glu Arg Arg Ile Ile Leu Leu Ala Phe
            100                 105                 110         
Gly Val Glu Leu Val Leu Thr Asp Pro Ala Lys Gly Met Lys Gly Ala
        115                 120                 125             
Ile Ala Lys Ala Glu Glu Ile Leu Ala Lys Thr Pro Asn Gly Tyr Met
    130                 135                 140                 
Leu Gln Gln Phe Glu Asn Pro Ala Asn Pro Lys Ile His Tyr Glu Thr
145                 150                 155                 160 
Thr Gly Pro Glu Ile Trp Lys Gly Thr Gly Gly Lys Ile Asp Gly Phe
                165                 170                 175     
Val Ser Gly Ile Gly Thr Gly Gly Thr Ile Thr Gly Ala Gly Lys Tyr
            180                 185                 190         
Leu Lys Glu Gln Asn Ala Asn Val Lys Leu Tyr Gly Val Glu Pro Val
        195                 200                 205             
Glu Ser Ala Ile Leu Ser Gly Gly Lys Pro Gly Pro His Lys Ile Gln
    210                 215                 220                 
Gly Ile Gly Ala Gly Phe Ile Pro Ser Val Leu Asn Val Asp Leu Ile
225                 230                 235                 240 
Asp Glu Val Val Gln Val Ser Ser Asp Glu Ser Ile Asp Met Ala Arg
                245                 250                 255     
Gln Leu Ala Leu Lys Glu Gly Leu Leu Val Gly Ile Ser Ser Gly Ala
            260                 265                 270         
Ala Ala Ala Ala Ala Ile Lys Leu Ala Gln Arg Pro Glu Asn Ala Gly
        275                 280                 285             
Lys Leu Phe Val Ala Ile Phe Pro Ser Phe Gly Glu Arg Tyr Leu Ser
    290                 295                 300                 
Thr Val Leu Phe Asp Ala Thr Arg Lys Glu Ala Glu Ala Met Thr Phe
305                 310                 315                 320 
Glu Ala
        


<210> 2
<211> 322
<212> PRT
<213> Arabiodopsis thaliana

<400> 2
Met Ala Ser Arg Ile Ala Lys Asp Val Thr Glu Leu Ile Gly Asn Thr
 1               5                  10                  15      
Pro Leu Val Tyr Leu Asn Asn Val Ala Glu Gly Cys Val Gly Arg Val
            20                  25                  30          
Ala Ala Lys Leu Glu Met Met Glu Pro Cys Ser Ser Val Lys Asp Arg
        35                  40                  45              
Ile Gly Phe Ser Met Ile Ser Asp Ala Glu Lys Lys Gly Leu Ile Lys
    50                  55                  60                  
Pro Gly Glu Ser Val Leu Ile Glu Pro Thr Ser Gly Asn Thr Gly Val
65                  70                  75                  80  
Gly Leu Ala Phe Thr Ala Ala Ala Lys Gly Tyr Lys Leu Ile Ile Thr
                85                  90                  95      
Met Pro Ala Ser Met Ser Thr Glu Arg Arg Ile Ile Leu Leu Ala Phe
            100                 105                 110         
Gly Val Glu Leu Val Leu Thr Asp Pro Ala Lys Gly Met Lys Gly Ala
        115                 120                 125             
Ile Ala Lys Ala Glu Glu Ile Leu Ala Lys Thr Pro Asn Gly Tyr Met
    130                 135                 140                 
Leu Gln Gln Phe Glu Asn Pro Ala Asn Pro Lys Ile His Tyr Glu Thr
145                 150                 155                 160 
Thr Gly Pro Glu Ile Trp Lys Gly Thr Gly Gly Lys Ile Asp Gly Phe
                165                 170                 175     
Val Ser Gly Ile Gly Thr Gly Gly Thr Ile Thr Gly Ala Gly Lys Tyr
            180                 185                 190         
Leu Lys Glu Gln Asn Ala Asn Val Lys Leu Tyr Gly Val Glu Pro Val
        195                 200                 205             
Glu Ser Ala Ile Leu Ser Gly Gly Lys Pro Gly Pro His Lys Ile Gln
    210                 215                 220                 
Gly Ile Gly Ala Gly Phe Ile Pro Ser Val Leu Asn Val Asp Leu Ile
225                 230                 235                 240 
Asp Glu Val Val Gln Val Ser Ser Asp Glu Ser Ile Asp Met Ala Arg
                245                 250                 255     
Gln Leu Ala Leu Lys Glu Gly Leu Leu Val Gly Ile Ser Ser Gly Ala
            260                 265                 270         
Glu Ala Ala Ala Ala Ile Lys Leu Ala Gln Arg Pro Glu Asn Ala Gly
        275                 280                 285             
Lys Leu Phe Val Ala Ile Phe Pro Ser Phe Gly Glu Arg Tyr Leu Ser
    290                 295                 300                 
Thr Val Leu Phe Asp Ala Thr Arg Lys Glu Ala Glu Ala Met Thr Phe
305                 310                 315                 320 
Glu Ala
        


<210> 3
<211> 324
<212> PRT
<213> Arabidopsis thaliana

<400> 3
Met Ala Ser Arg Ile Ala Lys Asp Val Thr Glu Leu Ile Gly Asn Thr
 1               5                  10                  15      
Pro Leu Val Tyr Leu Asn Asn Val Ser Leu Lys Gly Cys Val Gly Arg
            20                  25                  30          
Val Ala Ala Lys Leu Glu Met Met Glu Pro Cys Ser Ser Val Ser Val
        35                  40                  45              
Lys Asp Arg Ile Gly Phe Ser Met Ile Ser Asp Ala Glu Lys Lys Gly
    50                  55                  60                  
Leu Ile Lys Gln Gly Glu Ser Val Leu Ile Glu Pro Thr Ser Gly Asn
65                  70                  75                  80  
Thr Gly Glu Leu Leu Leu Ala Phe Thr Ala Ala Cys Lys Gly Tyr Lys
                85                  90                  95      
Leu Ile Ile Thr Met Pro Ala Ser Met Ser Thr Glu Arg Arg Ile Ile
            100                 105                 110         
Leu Leu Ala Phe Gly Val Glu Val Val Leu Thr Asp Pro Ala Lys Gly
        115                 120                 125             
Met Lys Gly Ser Tyr Arg Lys Ala Gln Glu Ile Leu Ala Lys Thr Pro
    130                 135                 140                 
Asn Gly Tyr Met Leu Gln Gln Phe Glu Asn Pro Ala Asn Pro Glu Ile
145                 150                 155                 160 
His Tyr Glu Pro Thr Gly Pro Glu Ile Trp Lys Gly Thr Gly Ala Lys
                165                 170                 175     
Ile Asp Gly Phe Val Ser Gly Ile Gly Thr Gly Gly Thr Ile Thr Gly
            180                 185                 190         
Ala Gly Lys Tyr Leu Lys Glu Gln Asn Pro Asn Phe Lys Leu Tyr Gly
        195                 200                 205             
Val Glu Pro Val Glu Ser Ala Ile Leu Ser Gly Gly Lys Pro Gly Pro
    210                 215                 220                 
Thr Lys Ile Gln Gly Ile Gly Ala Gly Phe Ile Pro Ser Val Leu Asn
225                 230                 235                 240 
Val Asp Leu Ile Asp Glu Val Val Gln Val Ser Ser Asp Glu Ser Ile
                245                 250                 255     
Asp Met Ala Arg Gln Leu Ala Leu Lys Glu Gly Leu Leu Val Gly Ile
            260                 265                 270         
Ser Ser Gly Ala Ala Ala Ala Ile Lys Leu Ala Gln Arg Pro Glu Asn
        275                 280                 285             
Ala Gly Lys Leu Phe Val Ala Ile Phe Pro Ser Phe Gly Arg Arg Tyr
    290                 295                 300                 
Leu Ser Thr Tyr Leu Phe Asp Ala Asp Lys Glu Arg Ser Arg Asn Met
305                 310                 315                 320 
Pro Ser Arg Pro
                


<210> 4
<211> 368
<212> PRT
<213> Arabidopsis thaliana

<400> 4
Met Ala Ser Val Ser Arg Arg Leu Leu Arg Arg Glu Thr Ile Pro Cys
 1               5                  10                  15      
Phe Ser His Thr Val Arg Lys Leu Phe Ser Thr Val Gly Ser Pro Ser
            20                  25                  30          
Phe Ala Gln Arg Leu Arg Asp Leu Pro Lys Asp Phe Pro Ser Thr Asn
        35                  40                  45              
Ala Lys Arg Asp Ala Ser Leu Leu Ile Gly Lys Thr Pro Leu Val Phe
    50                  55                  60                  
Leu Asn Lys Val Thr Glu Gly Cys Glu Ala Tyr Val Ala Ala Lys Gln
65                  70                  75                  80  
Glu His Phe Gln Pro Thr Cys Ser Ile Lys Asp Arg Pro Ala Ile Ala
                85                  90                  95      
Met Ile Ala Asp Ala Glu Lys Lys Lys Leu Ile Ile Pro Gly Lys Thr
            100                 105                 110         
Thr Leu Ile Glu Pro Thr Ser Gly Asn Met Gly Ile Ser Leu Ala Phe
        115                 120                 125             
Met Ala Ala Met Lys Gly Tyr Arg Ile Ile Met Thr Met Pro Ser Tyr
    130                 135                 140                 
Thr Ser Leu Glu Arg Arg Val Thr Met Arg Ser Phe Gly Ala Glu Leu
145                 150                 155                 160 
Val Leu Thr Asp Pro Ala Lys Gly Met Gly Gly Thr Val Lys Lys Ala
                165                 170                 175     
Tyr Asp Leu Leu Asp Ser Thr Pro Asp Ala Phe Met Cys Gln Gln Phe
            180                 185                 190         
Ala Asn Pro Ala Asn Thr Gln Ile His Phe Asp Thr Thr Gly Pro Glu
        195                 200                 205             
Ile Trp Glu Asp Thr Leu Gly Asn Val Asp Ile Phe Val Met Gly Ile
    210                 215                 220                 
Gly Ser Gly Gly Thr Val Ser Gly Val Gly Arg Tyr Leu Lys Ser Lys
225                 230                 235                 240 
Asn Pro Asn Val Lys Ile Tyr Gly Val Glu Pro Ala Glu Ser Asn Ile
                245                 250                 255     
Leu Asn Gly Gly Lys Pro Gly Pro His Ala Ile Thr Gly Asn Gly Val
            260                 265                 270         
Gly Phe Lys Pro Glu Ile Leu Asp Met Asp Val Met Glu Ser Val Leu
        275                 280                 285             
Glu Val Ser Ser Glu Asp Ala Ile Lys Met Ala Arg Glu Leu Ala Leu
    290                 295                 300                 
Lys Glu Gly Leu Met Val Gly Ile Ser Ser Gly Ala Asn Thr Val Ala
305                 310                 315                 320 
Ala Ile Arg Leu Ala Lys Met Pro Glu Asn Lys Gly Lys Leu Ile Val
                325                 330                 335     
Thr Ile His Ala Ser Phe Gly Glu Arg Tyr Leu Ser Ser Val Leu Phe
            340                 345                 350         
Asp Glu Leu Arg Lys Glu Ala Glu Glu Met Lys Pro Val Ser Val Asp
        355                 360                 365             


<210> 5
<211> 368
<212> PRT
<213> Arabidopsis thaliana

<400> 5
Met Ala Ser Val Ser Arg Arg Leu Leu Arg Arg Glu Thr Ile Pro Cys
 1               5                  10                  15      
Phe Ser His Thr Val Arg Lys Leu Phe Ser Thr Val Gly Ser Pro Ser
            20                  25                  30          
Phe Ala Gln Arg Leu Arg Asp Leu Pro Lys Asp Phe Pro Ser Thr Asn
        35                  40                  45              
Ala Lys Arg Asp Ala Ser Leu Leu Ile Gly Lys Thr Pro Leu Val Phe
    50                  55                  60                  
Leu Asn Lys Val Thr Glu Gly Cys Glu Ala Tyr Val Ala Ala Lys Gln
65                  70                  75                  80  
Glu His Phe Gln Pro Thr Cys Ser Ile Lys Asp Arg Pro Ala Ile Ala
                85                  90                  95      
Met Ile Ala Asp Ala Glu Lys Lys Lys Leu Ile Ile Pro Gly Lys Thr
            100                 105                 110         
Thr Leu Ile Glu Pro Thr Ser Gly Asn Met Gly Ile Ser Leu Ala Phe
        115                 120                 125             
Met Ala Ala Met Lys Gly Tyr Arg Ile Ile Met Thr Met Pro Ser Tyr
    130                 135                 140                 
Thr Ser Leu Glu Arg Arg Val Thr Met Arg Ser Phe Gly Ala Glu Leu
145                 150                 155                 160 
Val Leu Thr Asp Pro Ala Lys Gly Met Gly Gly Thr Val Lys Lys Ala
                165                 170                 175     
Tyr Asp Leu Leu Asp Ser Thr Pro Asp Ala Phe Met Cys Gln Gln Phe
            180                 185                 190         
Ala Asn Pro Ala Asn Thr Gln Ile His Phe Asp Thr Thr Gly Pro Glu
        195                 200                 205             
Ile Trp Glu Asp Thr Leu Gly Asn Val Asp Ile Phe Val Met Gly Ile
    210                 215                 220                 
Gly Ser Gly Gly Thr Val Ser Gly Val Gly Arg Tyr Leu Lys Ser Lys
225                 230                 235                 240 
Asn Pro Asn Val Lys Ile Tyr Gly Val Glu Pro Ala Glu Ser Asn Ile
                245                 250                 255     
Leu Asn Gly Gly Lys Pro Gly Pro His Ala Ile Thr Gly Asn Gly Val
            260                 265                 270         
Gly Phe Lys Pro Glu Ile Leu Asp Met Asp Val Met Glu Ser Val Leu
        275                 280                 285             
Glu Val Ser Ser Glu Asp Ala Ile Lys Met Ala Arg Glu Leu Ala Leu
    290                 295                 300                 
Lys Glu Gly Leu Met Val Gly Ile Ser Ser Gly Ala Asn Thr Val Ala
305                 310                 315                 320 
Ala Ile Arg Leu Ala Lys Met Pro Glu Asn Lys Gly Lys Leu Ile Val
                325                 330                 335     
Thr Ile His Ala Ser Phe Gly Glu Arg Tyr Leu Ser Ser Val Leu Phe
            340                 345                 350         
Asp Glu Leu Arg Lys Glu Ala Glu Glu Met Lys Pro Val Ser Val Asp
        355                 360                 365             


<210> 6
<211> 324
<212> PRT
<213> Arabiodopsis thaliana

<400> 6
Met Glu Glu Asp Arg Cys Ser Ile Lys Asp Asp Ala Thr Gln Leu Ile
 1               5                  10                  15      
Gly Asn Thr Pro Met Val Tyr Leu Asn Asn Ile Val Asp Gly Cys Val
            20                  25                  30          
Ala Arg Ile Ala Ala Lys Leu Glu Met Met Glu Pro Cys Ser Ser Val
        35                  40                  45              
Lys Glu Arg Ile Ala Tyr Gly Met Ile Lys Asp Ala Glu Asp Lys Gly
    50                  55                  60                  
Leu Ile Thr Pro Gly Lys Ser Thr Leu Ile Glu Ala Thr Ser Gly Asn
65                  70                  75                  80  
Thr Gly Ile Gly Leu Ala Phe Ile Gly Ala Ala Lys Gly Tyr Lys Val
                85                  90                  95      
Val Leu Thr Met Pro Ser Ser Met Ser Leu Glu Arg Lys Ile Ile Leu
            100                 105                 110         
Leu Ala Leu Gly Ala Glu Val His Leu Thr Asp Pro Ser Lys Gly Val
        115                 120                 125             
Gln Gly Ile Ile Asp Lys Ala Glu Glu Ile Cys Ser Lys Asn Pro Asp
    130                 135                 140                 
Ser Ile Met Leu Glu Gln Phe Lys Asn Pro Ser Asn Pro Gln Thr His
145                 150                 155                 160 
Tyr Arg Thr Thr Gly Pro Glu Ile Trp Arg Asp Ser Ala Gly Glu Val
                165                 170                 175     
Asp Ile Leu Val Ala Gly Val Gly Thr Gly Gly Thr Leu Ser Gly Ser
            180                 185                 190         
Gly Arg Phe Leu Lys Glu Lys Asn Lys Asp Phe Lys Val Tyr Gly Val
        195                 200                 205             
Glu Pro Thr Glu Ser Ala Val Ile Ser Gly Gly Lys Pro Gly Thr His
    210                 215                 220                 
Leu Ile Gln Gly Ile Gly Ala Gly Leu Ile Pro Asp Asn Leu Asp Phe
225                 230                 235                 240 
Asn Val Leu Asp Glu Val Ile Gln Val Thr Ser Val Glu Ala Ile Glu
                245                 250                 255     
Thr Ala Lys Leu Leu Ala Leu Lys Glu Gly Leu Leu Val Gly Ile Ser
            260                 265                 270         
Ser Gly Ala Ala Ala Ala Ala Ala Ile Lys Val Ala Lys Arg Pro Glu
        275                 280                 285             
Asn Ala Gly Lys Leu Ile Val Val Ile Phe Pro Ser Gly Gly Glu Arg
    290                 295                 300                 
Tyr Leu Ser Thr Ser Leu Phe Glu Ser Val Arg His Glu Ala Glu Asn
305                 310                 315                 320 
Leu Pro Ile Gln
                

